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Ursprungszeugnis (CoO)/Analysenzertifikate (CoA)

OGS1879

PSF-TEF1-NH2-INTS - INVERTASE SECRETION PLASMID

Name: PSF-TEF1-NH2-INTS - INVERTASE SECRETION PLASMID
Brand: SIGMA

plasmid vector for molecular cloning

Synonym(e):

cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector

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About This Item

UNSPSC-Code:

12352200

NACRES:

NA.85

Form

buffered aqueous solution

Mol-Gew.

size 6787 bp

Bakterienauswahl

kanamycin

Replikationsursprung

2Micron
pUC (500 copies)

Peptidspaltung

no cleavage

Lage der Peptid-Tags

N-terminal

Promoter

Promoter name: TEF1
Promoter activity: constitutive
Promoter type: yeast

Reportergen

none

Sekretionssignal

alpha factor FL

Versandbedingung

ambient

Lagertemp.

−20°C

Hefeauswahl

uracil

Allgemeine Beschreibung

This plasmid is designed to express tagged proteins in yeast cells (Saccharomyces cerevisiae). The plasmid contains an auxotrophic Uracil selection expression cassette (URA3) that allows for the positive selection of yeast that are deficient in the URA3 gene (YEL021W). This is typically achieved by growing the yeast in minimal media that is reconstituted with the essential amino acids and nucleotides but excluding Uracil.

About the Peptide Tag:This plasmid contains an Invertase (Ints) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases.

Promoter Expression Level: This plasmid contains the Yeast Elongation Factor Alpha promoter (TEF1). This is the strongest of the yeast promoters that we sell. It is a constitutive promoter and requires no induction. If you are interested in weaker promoters levels than we also stock plasmids that contain the following promoters in order of decreasing strength TPI (strong), ADHI (medium), STE5 (weak). We also stock Galactose inducible promoter plasmids if inducible expression is required. Please contact us for further information.

Sequenz

To view sequence information for this product, please visit the product page

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Lagerklassenschlüssel

12 - Non Combustible Liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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The GTP- and Phospholipid-Binding Protein TTD14 Regulates Trafficking of the TRPL Ion Channel in Drosophila Photoreceptor Cells.

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