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Merck

N6910

Sigma-Aldrich

Neomycin -trisulfat (Salz) Hydrat

Biotechnology Performance Certified, suitable for cell culture

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About This Item

Empirische Formel (Hill-System):
C23H46N6O13 · 3H2SO4 · xH2O
CAS-Nummer:
Molekulargewicht:
908.88 (anhydrous basis)
EG-Nummer:
UNSPSC-Code:
51101500
PubChem Substanz-ID:

Qualität

Biotechnology Performance Certified

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Löslichkeit

H2O: 50 mg/mL

Wirkungsspektrum von Antibiotika

Gram-negative bacteria
Gram-positive bacteria

Wirkungsweise

protein synthesis | interferes

SMILES String

O.OS(O)(=O)=O.OS(O)(=O)=O.OS(O)(=O)=O.NC[C@@H]1O[C@H](O[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)O[C@@H]3[C@@H](O)[C@H](N)C[C@H](N)[C@H]3O[C@H]4O[C@H](CN)[C@@H](O)[C@H](O)[C@H]4N)C(N)[C@@H](O)[C@@H]1O

InChI

1S/C23H46N6O13.3H2O4S.H2O/c24-2-7-13(32)15(34)10(28)21(37-7)40-18-6(27)1-5(26)12(31)20(18)42-23-17(36)19(9(4-30)39-23)41-22-11(29)16(35)14(33)8(3-25)38-22;3*1-5(2,3)4;/h5-23,30-36H,1-4,24-29H2;3*(H2,1,2,3,4);1H2/t5-,6+,7-,8+,9-,10-,11?,12+,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23+;;;;/m1..../s1

InChIKey

WHAGUNPVKDUVFV-QGTTWHFQSA-N

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Allgemeine Beschreibung

Chemical structure: aminoglycoside

Anwendung

Neomycin Trisulfate is produced by Streptomyces containing a minimum of 85% neomycin B. It has been used as a selection agent for prokaryotic cells transformed using the neo selectable marker gene, and to study ototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts, and extraction of Nuclear Phosphatidylinositol 4,5-Bisphosphate-Interacting Proteins †††. It is recommended for use in cell culture applications at 50 mg/L. This product is biotechnology performance certified and cell culture tested.

Biochem./physiol. Wirkung

Wirkungsweise: Dieses Produkt wirkt, indem es an die 30S- und 50S-Untereinheiten bindet, eine Fehlcodierung hervorruft und die Initiation und Elongation der Proteinsynthese hemmt. Neomycin blockiert auch die spannungsempfindlichen Ca2+-Kanäle, ohne die Na+/Ca2+-Antiporter in den Neuronen zu beeinträchtigen.

Antimikrobielles Spektrum: Neomycin wirkt gegen grampositive und gramnegative Bakterien.

Vorsicht

Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.

Angaben zur Herstellung

Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.

Sonstige Hinweise

Keep container tightly closed in a dry and well-ventilated place.

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1 - Skin Sens. 1

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Die Dokumentenbibliothek aufrufen

F S Vassbotn et al.
The Journal of biological chemistry, 267(22), 15635-15641 (1992-08-05)
The aminoglycoside neomycin has recently been found to affect certain platelet-derived growth factor (PDGF) responses in C3H/10T1/2 C18 fibroblasts. Using porcine aortic endothelial cells transfected with PDGF alpha- or beta-receptors, we explored the possibility that neomycin interferes with the interaction
Michael G Leitner et al.
Molecular pharmacology, 79(1), 51-60 (2010-10-12)
Aminoglycoside antibiotics (AGs) are severely ototoxic. AGs cause degeneration of outer hair cells (OHCs), leading to profound and irreversible hearing loss. The underlying mechanisms are not fully understood. OHC survival critically depends on a specific K+ conductance (I(K,n)) mediated by
Aurélia E Lewis et al.
Molecular & cellular proteomics : MCP, 10(2), M110-M110 (2010-11-05)
Considerable insight into phosphoinositide-regulated cytoplasmic functions has been gained by identifying phosphoinositide-effector proteins. Phosphoinositide-regulated nuclear functions however are fewer and less clear. To address this, we established a proteomic method based on neomycin extraction of intact nuclei to enrich for
Sung Ryeol Park et al.
Natural product reports, 30(1), 11-20 (2012-11-28)
The 2-deoxystreptamine-containing aminoglycosides, such as neomycin, kanamycin and gentamicin, are an important class of antibiotics. A detailed understanding of the complete biosynthetic pathway of aminoglycosides and their biosynthetic enzymes will allow us to not only generate more robust antibiotic agents
Wenlin Zhou et al.
Biology of reproduction, 87(6), 144-144 (2012-10-27)
The domesticated silkworm Bombyx mori L. has important roles in basic biological research and applied science. To explore the practical use of transgenic technology in agricultural silkworm varieties, we fused the neomycin-resistance gene (Neo(R)) and the green fluorescent protein gene

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