N4645

4-Nitrophenylphosphat Dinatriumsalz Hexahydrat

powder, BioReagent, suitable for cell culture, ≥97%

• Synonym(e): p-Nitrophenylphosphat Dinatriumsalz, Dinatrium-4-nitrophenylphosphat, Phosphatase-Substrat, Phosphorsäure-mono-(4-nitrophenylester) Hexahydrat, pNPP Dinatrium Hexahydrat
• Lineare Formel: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• CAS-Nummer: 333338-18-4
• Molekulargewicht: 371.14
• Beilstein: 5324661
• EG-Nummer: 224-246-5
• MDL-Nummer: MFCD00066288
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24897708
• NACRES: NA.75

Eigenschaften

• Biologische Quelle: synthetic (organic)
• Qualitätsniveau: 200
• Produktlinie: BioReagent
• Assay: ≥97%
• Form: powder
• Methode(n): cell culture | mammalian: suitable
• Löslichkeit: H₂O: 100 mg/mL
• Lagertemp.: −20°C
• SMILES String: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• InChIKey: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Beschreibung

Allgemeine Beschreibung

Chromogenes Substrat für die Bestimmung von sauren und alkalischen Phosphatasen. Die Reaktion erzeugt ein lösliches gelbes Endprodukt. Die Reaktion kann mit 3 N NaOH gestoppt und bei 405 nm abgelesen werden.

Anwendung

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:

• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
• to treat adherent cells in adhesion assay
• as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA

Biochem./physiol. Wirkung

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)