Yes, it can be used on formalin-fixed tissue, but one would need to amplify the gene of interest before bisulfite treatment
MOD50
Impressum® DNA-Modifizierungskit
For bisulfite DNA conversion & purification
Synonym(e):
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Über diesen Artikel
UNSPSC Code:
41116158
EC Number:
231-548-0
NACRES:
NA.54
Fortfahren mit
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenVerwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| usage sufficient for 50 reactions | usage - | usage - | usage - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| storage temp. 20-25°C | storage temp. −20°C | storage temp. - | storage temp. −20°C |
General description
The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
Application
Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.
Features and Benefits
- Only 50 picograms of DNA or 20 cells are required
- Procedure takes less than 2 hours
- Greater than 99% conversion rate
- Extremely low degradation
- Option of convenient one-step protocol
- Consistent and reproducible Bisulfite Modification
- Can be used with genomic, endonuclease digested, and FFPE DNA
Preparation Note
All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.
Legal Information
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit-Komponenten auch einzeln erhältlich
Produkt-Nr.
Beschreibung
SDB
- T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, natural
signalword
Danger
supp_hazards
Lagerklasse
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
The elephant shark methylome reveals conservation of epigenetic regulation across jawed vertebrates
Peat JR, et al.
F1000Research (2017)
Sarah Rose Huffman et al.
Methods in molecular biology (Clifton, N.J.), 925, 201-209 (2012-08-22)
Researchers whose experimental models are mammalian oocytes and preimplantation embryos are often limited by the yield of nucleic acids that can be isolated from such a small sample size. In addition, the limited number of cells from these types of
Julian R Peat et al.
Methods in molecular biology (Clifton, N.J.), 1708, 161-169 (2017-12-11)
The epigenetic mark 5-methylcytosine confers heritable regulation of gene expression that can be dynamically modulated during transitions in cell fate. With the development of high-throughput sequencing technologies, it is now possible to obtain comprehensive genome-wide maps of the mammalian DNA
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
Artikel
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
Protokolle
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Verwandter Inhalt
The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.
-
Can Product MOD50, Imprint® DNA Modification Kit, be used on formalin-fixed tissue ?
1 Antwort-
Hilfreich?
-
-
How many reactions can be run with Product MOD50, Imprint® DNA Modification Kit?
1 Antwort-
This kit can be used for 50 reactions.
Hilfreich?
-
-
Can I use Product MOD50, Imprint® DNA Modification Kit with DNA templates that contain high GC content or secondary structures?
1 Antwort-
Yes, but it will be necessary to increase the bisulfite reaction time to 150 to 180 min.
Hilfreich?
-
-
What is the minimum amount of DNA that can be used with Product MOD50, Imprint® DNA Modification Kit?
1 Antwort-
DNA amounts ranging from 0.1 ng to 1 mg have been used successfully. For optimal modification, the DNA quantity should be in the range of 50-200 ng.
Hilfreich?
-
-
Can you store the DNA modified using Product MOD50, Imprint® DNA Modification Kit?
1 Antwort-
The modified DNA generated with this kit can be stored for 2 months at -20 °C, 6 months at -80 °C.
Hilfreich?
-
-
What is the Department of Transportation shipping information for this product?
1 Antwort-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Hilfreich?
-
Aktive Filter
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung