MAK075
Ascorbic Acid Assay Kit II
sufficient for 100 colorimetric tests
Synonym(e):
FRASC Assay, Ferric Reducing/Antioxidant and Ascorbic Acid
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About This Item
UNSPSC-Code:
12161503
NACRES:
NA.84
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Verwendung
sufficient for 100 colorimetric tests
Nachweisverfahren
colorimetric
Lagertemp.
−20°C
Allgemeine Beschreibung
The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.
Anwendung
Ascorbic Acid Assay Kit II has been used to determine ascorbic acid concentration in vitreous sample from the eye.
Eignung
Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.
Prinzip
In this assay, ascorbic acid concentration is determined using the Ferric Reducing/Antioxidant and Ascorbic Acid (FRASC) assay. Fe3+ is reduced to Fe2+ by antioxidants present in the sample, which results in a colorimetric (593 nm) product. The addition of ascorbate oxidase to parallel samples oxidizes any ascorbic acid present allowing for the measurement of the ascorbic acid concentration.
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10 - Combustible liquids
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Karthik Murali et al.
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