M9818

Anti-Myopodin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL-Nummer: MFCD06798472
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 80 kDa
• Speziesreaktivität: rat
• Methode(n): immunohistochemistry (frozen sections): 1-2 μg/mL using rat skeletal muscle and rat kidney; western blot: 1-2 μg/mL using cytosolic fraction of rat skeletal muscle
• UniProt-Hinterlegungsnummer: D4A702
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SYNPO2(171024) ; mouse ... Synpo2(118449) ; rat ... Synpo2(499702)

Beschreibung

Allgemeine Beschreibung

Myopodin (80-95 kDa), a novel actin bundling protein, is an additional member of the synaptopodin gene family. Myopodin is expressed in skeletal and cardiac muscles. Myopodin contains one PPXY motif, multiple PXXP motifs, and a nuclear export sequence (NES). In the-disc, myopodin colocalizes with α-actinin. Myopodin, like several actin-bundling proteins, has been shown to shuttle between the nucleus and cytoplasm. It is localized in nucleus in myoblasts.

Immunogen

synthetic peptide encoding amino acids 566-585 located at the mid-region of human myopodin, conjugated to KLH. This sequence is highly conserved (77% sequence identity) in mouse myopodin and is not found in human or rat synaptopodin.

Anwendung

Anti-Myopodin has been used in:

• immunohistochemistry
• immunostaining
• western blot analysis

Biochem./physiol. Wirkung

Myopodin directly binds to actin and contains an actin-binding site in the centre of the protein. Myopodin has actin bundling activity as shown by lantraculin - a sensitive cytosolic actin bundles and nuclear actin loops in transfected cells expressing GFP-myopodin. It binds to stress fibres in a punctuated pattern into the Z-disc during myotube differentiation. Myopodin is frequently down-regulated in invasive stages of some types of cancers, including invasive bladder tumors. Frequent complete or partial deletions of the myopodin gene have been shown to occur in 80% of invasive prostate cancer cases. Expression of myopodin induces suppression of tumor growth both in vivo and in vitro. Myopodin inhibits tumor metastasis by functioning as a tumor suppressor gene.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable