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M6552

Sigma-Aldrich

Monoclonal Anti-Matrix Metalloproteinase-3 antibody produced in mouse

clone SL-1 IIIC4, purified immunoglobulin, buffered aqueous solution

Synonym(e):

Anti-MMP-3

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About This Item

MDL-Nummer:
MFCD01094317
UNSPSC-Code:
12352203

Biologische Quelle

mouse

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

SL-1 IIIC4, monoclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~48 kDa (active form)
antigen ~57 kDa (latent form)

Speziesreaktivität

human

Methode(n)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using heat/pressure cooker pre-treatment
immunohistochemistry (frozen sections): 1:50-1:100
immunoprecipitation (IP): 10 using protein lysate (μL/mg)
indirect immunofluorescence: 4-5 μg/mL
western blot: 1:200-1:400 using conditioned medium from PMA-treated HT-1080 cells or bladder, breast, or ovarian carcinoma

Isotyp

IgG1

UniProt-Hinterlegungsnummer

P08254

Versandbedingung

dry ice

Lagertemp.

−20°C

Angaben zum Gen

human ... MMP3(4314)

Immunogen

APMA-activated human matrix metalloproteinase-3 (stromelysin-1).

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Physikalische Form

Solution in 10 mM phosphate buffered saline and 0.2% bovine serum albumin, pH 7.4, containing 0.1% sodium azide.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
107K1213
104K1568
112K0700
083K1034
012K0251

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Die Dokumentenbibliothek aufrufen

Toru Ishibashi et al.
International immunopharmacology, 21(2), 468-473 (2014-06-15)
The aim of this study was to demonstrate the safety and efficacy of H2-saline infusion for treatment of rheumatoid arthritis (RA). We conducted a randomized, double-blind, placebo-controlled investigation of the infusion of 1 ppm H2-dissolved saline (H2-saline) in 24 RA
Thangarajan Rajkumar et al.
BMC cancer, 11, 80-80 (2011-02-23)
Cervical cancer is the most common cancer among Indian women. This cancer has well defined pre-cancerous stages and evolves over 10-15 years or more. This study was undertaken to identify differentially expressed genes between normal, dysplastic and invasive cervical cancer.
Brenda J Tripathi et al.
Experimental eye research, 78(3), 653-660 (2004-04-27)
We investigated the mRNA and protein expression of fibronectin and stromelysin-1 (matrix metalloproteinase-3, MMP-3) by trabecular cells treated with growth factors present in primary and secondary aqueous humors. Serum-deprived trabecular cells were incubated for 48 hr or 7 days in
W Brian Saunders et al.
Journal of cell science, 118(Pt 10), 2325-2340 (2005-05-05)
Previous work has shown that endothelial cell (EC)-derived matrix metalloproteinases (MMPs) regulate regression of capillary tubes in vitro in a plasmin- and MMP-1 dependent manner. Here we report that a number of serine proteases can activate MMP-1 and cause capillary
Chuan Ma et al.
PloS one, 9(9), e107544-e107544 (2014-09-17)
To examine the possible involvement and regulatory mechanisms of extracellular signal-regulated kinase (ERK) pathway in the temporomandibular joint (TMJ) of rats subjected to chronic sleep deprivation (CSD). Rats were subjected to CSD using the modified multiple platform method (MMPM). The
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