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Merck

M6313

Sigma-Aldrich

Mimetisches Grün 1 Liganden-Affinitäts-Adsorbens

suspension

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About This Item

MDL-Nummer:
UNSPSC-Code:
23151817
NACRES:
NA.56

Form

suspension

Qualitätsniveau

Versandbedingung

wet ice

Lagertemp.

2-8°C

Anwendung

Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Pol from E. coli lysates as well as for the purification of other DNA polymerases.

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Gefahreneinstufungen

Flam. Liq. 3

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 1

Flammpunkt (°F)

95.0 °F - closed cup

Flammpunkt (°C)

35 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


Analysenzertifikate (COA)

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S C Woodcock et al.
The Biochemical journal, 313 ( Pt 2), 415-421 (1996-01-15)
CysG, also known as uroporphyrinogen III methylase and sirohaem synthase (CysG; EC 2.1.1.107), is a multifunctional enzyme that is able to transform uroporphyrinogen III into sirohaem via two S-adenosyl-L-methionine (AdoMet)-dependent transmethylations, an NAD(+)-dependent dehydrogenation and a ferrochelation. The apparent tight
B V Thage et al.
Journal of applied microbiology, 96(3), 593-602 (2004-02-14)
Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interaction chromatography. The enzyme was found
Michael Kotik et al.
Biochimica et biophysica acta, 1760(2), 245-252 (2005-12-14)
Purification of a novel enantioselective epoxide hydrolase from Aspergillus niger M200 has been achieved using ammonium sulphate precipitation, ionic exchange, hydrophobic interaction, and size-exclusion chromatography, in conjunction with two additional chromatographic steps employing hydroxylapatite, and Mimetic Green. The enzyme was
J Ni et al.
The Journal of biological chemistry, 272(16), 10853-10858 (1997-04-18)
A new member of the human cystatin superfamily, called cystatin E, has been found by expressed sequence tag (EST) sequencing in amniotic cell and fetal skin epithelial cell cDNA libraries. The sequence of a full-length amniotic cell cDNA clone contained
Sotirios Melissis et al.
Biotechnology journal, 2(1), 121-132 (2006-12-22)
The thermostable Thermus aquaticus DNA polymerase (Taq Pol) has been the key factor in transforming the initial PCR method into one with huge impact in molecular biology and biotechnology. Therefore, the development of effective affinity adsorbents for the purification of

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