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Merck

M5774

Sigma-Aldrich

Anti-Mouse Serum antibody produced in rabbit

whole antiserum

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

whole antiserum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Enthält

15 mM sodium azide

Speziesreaktivität

mouse

Methode(n)

immunoelectrophoresis: suitable

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Mouse serum comprises hormones, antigens, electrolytes and antibodies. It serves blocking agent in immunoassays.

Spezifität

Strong reactivity with normal mouse serum has been determined by immunoelectrophoresis (IEP). This antiserum has not been assayed for interspecies crossreactivity.

Anwendung

Anti-Mouse Serum antibody produced in rabbit has been used in differential fluorescent labelling. It has also been used in differential staining of inner cell mass (ICM) and and trophectoderm (TE) cells. It has been also been used in the removal of zona pellucidae for the isolation of inner cell mass (ICM) from mouse blastocysts.
Anti-Mouse Serum antibody produced in rabbit was used as blocking agent in differential cell count of trophectoderm and inner cell mass cells of mouse embryos.

Physikalische Form

Rabbit Anti-Mouse Serum is provided as a liquid containing 15 mM sodium azide as preservative.

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Die Dokumentenbibliothek aufrufen

Namfon Inna et al.
Clinical and experimental reproductive medicine, 45(3), 110-115 (2018-09-12)
To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group
Both the folate cycle and betaine-homocysteine methyltransferase contribute methyl groups for DNA methylation in mouse blastocysts
Zhang B, et al.
Faseb Journal, 29(3), 1069-1079 (2015)
J Van der Elst et al.
Human reproduction (Oxford, England), 13(6), 1595-1599 (1998-08-04)
We demonstrated previously that ultra-rapid freezing of mouse oocytes with 3.5 M dimethylsulphoxide (DMSO) decreased cell numbers in day 5 in-vitro cultured blastocysts. In the present study we counted cell numbers of trophectoderm (TE) and inner cell mass (ICM) separately
Carla Mulas et al.
Development (Cambridge, England), 146(6) (2019-03-28)
The power of mouse embryonic stem (ES) cells to colonise the developing embryo has revolutionised mammalian developmental genetics and stem cell research. This power is vulnerable, however, to the cell culture environment, deficiencies in which can lead to cellular heterogeneity
Raúl Fernández-González et al.
Reproduction (Cambridge, England), 137(2), 271-283 (2008-11-20)
We have reported that in vitro culture (IVC) of preimplantation mouse embryos in the presence of FCS produces long-term effects (LTE) on development, growth and behaviour of the offspring at adult age. To analyse the mechanisms underlying this phenomenon, we

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