L4542
Anti-phospho-Lck (pTyr505) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
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About This Item
Biologische Quelle
rabbit
Qualitätsniveau
Konjugat
unconjugated
Antikörperform
affinity isolated antibody
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Speziesreaktivität
human
Methode(n)
western blot: 0.1-1.0 μg/mL using full length recombinant human Lck protein.
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−70°C
Posttranslationale Modifikation Target
phosphorylation (pTyr505)
Angaben zum Gen
human ... LCK(3932)
mouse ... Lck(16818)
rat ... Lck(313050)
Allgemeine Beschreibung
The signalling in T cells is mediated by receptor and non-receptor protein tyrosine kinases (PTK). Among the family of non-receptors PTK, Lck is a lymphoid-specific cytosolic PTK essential for T-cell development and function. Lck is essential for selection and maturation of developing T cells in the thymus. It plays a significant role in T-cell receptors mediated signalling by constitutive association with CD4 and CD8 receptors. In addition, Lck undergoes phosphorylation and activation by association with CD2 molecule. Lck also plays a role in T-cell proliferation in response to IL-2 receptor pathway. The activity of Lck is known to be regulated by phosphorylation of two conserved tyrosine residues, tyrosine 505 (equivalent to tyrosine 529 in c-Src) and tyrosine 394 (equivalent to tyrosine 418 in c-Src). Phosphorylation at Tyr505 stabillizes Lck and renders it biologically inactive.
Anti-phospho Lck (pTyr505) specifically recognizes human Lck (pTyr505). Mouse (100% homology) and chicken (91.7% homology) are expected to react.
Anti-phospho Lck (pTyr505) specifically recognizes human Lck (pTyr505). Mouse (100% homology) and chicken (91.7% homology) are expected to react.
Immunogen
synthetic phosphopeptide derived from the region of Lck that is phosphorylated on tyrosine 505. Rat and mouse have 100% homology with human.
Anwendung
A working concentration of 0.1-1.0 μg/mL is recommended for detection of human Lck protein by immunoblotting.
Physikalische Form
Solution in phosphate buffer, pH 7.4, with 1 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Angaben zur Herstellung
The antibody is preadsorbed to remove any reactivity towards a non-phosphorylated Lck.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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10 - Combustible liquids
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Tahir M Razzaq et al.
Immunology, 113(4), 413-426 (2004-11-24)
There is now considerable evidence suggesting that the plasma membrane of mammalian cells is compartmentalized by functional lipid raft microdomains. These structures are assemblies of specialized lipids and proteins and have been implicated in diverse biological functions. Analysis of their
The lck tyrosine protein kinase.
B M Sefton
Oncogene, 6(5), 683-686 (1991-05-01)
N Isakov et al.
European journal of biochemistry, 267(12), 3413-3421 (2000-06-10)
Protein tyrosine kinases (PTKs) are critically involved in signaling pathways that regulate cell growth, differentiation, activation, and transformation. It is not surprising, therefore, that viruses acquire effector molecules targeting these kinases to ensure their own replication and/or persistence. This review
S J Anderson et al.
Immunology today, 16(2), 99-105 (1995-02-01)
Recent evidence supports the view that p56lck, a non-receptor protein tyrosine kinase, serves as the signaling element that 'senses' synthesis of a functional T-cell receptor beta chain, thereby promoting thymocyte maturation. Here, Steven Anderson and Roger Perlmutter review current data
C Couture et al.
The Journal of biological chemistry, 271(40), 24880-24884 (1996-10-04)
Src homology 2 (SH2) domains bind to phosphotyrosine (Tyr(P)) residues in specific sequence contexts in other proteins and thereby mediate tyrosine phosphorylationdependent protein-protein interactions. The SH2 domain of the Src family kinase Lck is phosphorylated at tyrosine 192 in T
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