L3397

Luria Bouillon (Miller Modifikation)

Powder microbial growth medium

• Synonym(e): Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• UNSPSC-Code: 41106200
• NACRES: NA.85

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Sterilität: non-sterile
• Form: powder
• Zusammensetzung: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• Methode(n): microbiological culture: suitable
• pH-Wert: 6.8-7.2(1.55% solution)
• Eignung: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• Anwendung(en): food and beverages; microbiology
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Anwendung

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Leistungsmerkmale und Vorteile

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

Angaben zur Herstellung

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Rekonstituierung

Herstellung
1. 15.5 g in 1 L destilliertem Wasser suspendieren.
2. Für 15 Minuten bei 121°C autoklavieren.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable