I9401

Interleukin-1β human

IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): IL-1β, Lymphocyte activating factor
• MDL-Nummer: MFCD00131399
• UNSPSC-Code: 12352202

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 200
• Rekombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤12 pg/mL ED₅₀
• Qualität: endotoxin tested
• Mol-Gew.: 17.3 kDa
• Verpackung: pkg of 5 and 25 μg; pkg of 25 μg
• Methode(n): cell culture | mammalian: suitable
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: P01584
• Lagertemp.: −20°C
• Angaben zum Gen: human ... IL1B(3553)

Beschreibung

Biochem./physiol. Wirkung

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cell lymphocytes, which then proliferate and secrete interleukin-2 (IL-2). IL-1 is released primarily from stimulated macrophages and monocytes, but has also been shown to be released from several other types and is thought to play a key role in inflammatory and immune responses. Other synonyms for IL-1 include: endogenous pyrogen (EP), mitogenic protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFM), B Cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF) The two closely related agents Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) share 62% homology in amino acid sequence and elicit nearly identical biological responses. IL-1α and IL-1β are both approximately 17 kDa with some heterogeneity in the amount of glycosylation.

Physikalische Form

Lyophilized from a 0.2 μm filtered buffered solution.

Hinweis zur Analyse

The biological activity is measured by the dose-dependent stimulation of MTS cleavage by murine D10S cells.

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)