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GE17-5279-02

Glutathione Seph High Performance

Cytiva 17-5279-02, pack of 100 mL

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About This Item

UNSPSC-Code:
23151817
NACRES:
NA.56

ligand

glutathione

Verpackung

pack of 100 mL

Hersteller/Markenname

Cytiva 17-5279-02

Matrix

highly cross-linked 6% agarose

Durchschnittliche Teilgröße

34 μm

cleaning

3-12

working range

3-12

Kapazität

>7 mg/mL binding capacity(medium)

Allgemeine Beschreibung

Glutathione Sepharose High Performance is an affinity medium for high resolution purification of Glutathione S transferase (GST) tagged proteins.

Anwendung

Glutathione Sepharose High Performance is used for the purification of GST-tagged Proteins from bacterial lysates and other S-transferases or glutathione-dependent Proteins. GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function.

Leistungsmerkmale und Vorteile

  • 34 μm bead size for improved efficiency and resolution
  • Fast, one-step purification of GST-tagged Proteins and other glutathione S-trasferase or glutathione-dependent Proteins
  • Mild conditions during the purification process preserves Protein antigenicity and function

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

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Protokolle

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

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