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GE17-5179-01

Sigma-Aldrich

Mono Q® 4.6/100 PE

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About This Item

UNSPSC-Code:
41115700
NACRES:
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2N+(CH3)3

Materialien

PEEK column

Beschreibung

Ion Exchanger Type (value)

Verpackung

pkg of 1 ea

Parameter

580 psi

Bettvolumen

1.7 mL

Säule ID

4.6 mm

Matrix

monodisperse porous polystyrene/divinyl benzene beads
polymeric

Aktive Matrixgruppe

ammonium ion, quaternary phase

Durchschnittliche Teilgröße

10 μm

cleaning

1-14

Arbeitsbereich

2-12

Trenntechnik

anion exchange

Allgemeine Beschreibung

Mono Q® is a strong anion exchanger prepacked with MonoBeads in a Tricorncolumn. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Leistungsmerkmale und Vorteile

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Lagerung und Haltbarkeit

Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Rechtliche Hinweise

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono Q is a registered trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

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Analysenzertifikate (COA)

Lot/Batch Number

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Artikel

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Protokolle

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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