G5518

Anti-Goat IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL-Nummer: MFCD00162334
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Methode(n): indirect ELISA: 1:50,000; quantitative precipitin assay: 3.5 mg/mL
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

IgG is a glycoprotein. It is a major class of immunoglobulin. IgG consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

Immunogen

Anti-Goat IgG is produced in rabbit using as immunogen purified goat IgG.

Anwendung

Anti-Goat IgG (whole molecule) antibody produced in rabbit has been used in chromatin immunoprecipitation (ChIP) assay and electron microscopy.

Anti-Goat IgG (whole molecule) antibody produced in rabbit was used in combination with protein A-colloidal gold conjugate in electron microscopy of in vitro matured and fertilized boar oocytes.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)

Biochem./physiol. Wirkung

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Lagerung und Haltbarkeit

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable