F6380
Anti-Human IgG (γ-chain specific)−FITC antibody produced in goat
IgG fraction of antiserum, buffered aqueous solution
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About This Item
Empfohlene Produkte
Biologische Quelle
goat
Konjugat
FITC conjugate
Antikörperform
IgG fraction of antiserum
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Methode(n)
direct immunofluorescence: 1:32
Lagertemp.
2-8°C
Posttranslationale Modifikation Target
unmodified
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Allgemeine Beschreibung
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Human IgG (γ-chain specific)-FITC antibody is specific for human IgG subclasses. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. Goat anti-human IgG is conjugated to Sigma Fluorescein Isothiocyanate (FITC).
Anti-Human IgG (γ-chain specific)-FITC antibody is specific for human IgG subclasses. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. Goat anti-human IgG is conjugated to Sigma Fluorescein Isothiocyanate (FITC).
Immunogen
Pooled normal human serum
Anwendung
Anti-Human IgG (γ-chain specific)-FITC antibodymay be used for immunofluorescent labelling of human peripheral blood lymphocytes at a working antibody dilution of 1:32 or 1:130. was used to determine IgG isotypes on human RBC by exhaustive photon reassignment microscopy.
Physikalische Form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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U Specks et al.
Clinical and experimental immunology, 109(2), 286-295 (1997-08-01)
We have expressed conformationally intact, enzymatically active recombinant PR3 in HMC-1 cells (HMC-1/PR3 cells) that is recognized by C-ANCA. Here we directly compared the clinical utility of C-ANCA testing by indirect immunofluorescence (IIF) using HMC-1/PR3 cell cytospin versus polymorphonuclear neutrophil
Monil Singhai et al.
Tropical parasitology, 12(1), 54-58 (2022-08-05)
A range of assays have been developed to detect specific antileishmanial antibody, such as rK 39 immunochromatographic test (ICT), KE 16 ICT, ELISA test, and indirect immunofluorescent antibody test (IFAT), which play a crucial role in serological diagnosis of visceral
J Gysin et al.
Infection and immunity, 67(12), 6596-6602 (1999-11-24)
We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and
Ingrid J G Burvenich et al.
mAbs, 8(4), 775-786 (2016-04-01)
IgG has a long half-life through engagement of its Fc region with the neonatal Fc receptor (FcRn). The FcRn binding site on IgG1 has been shown to contain I253 and H310 in the CH2 domain and H435 in the CH3
S Hashira et al.
Pediatrics international : official journal of the Japan Pediatric Society, 42(4), 337-342 (2000-09-15)
Maternal immunoglobulin G (IgG), transferred across the placenta to the fetus during intrauterine life, is an important component of the neonatal immunological defence mechanisms against infection. There is controversy with respect to differences in placental transfer of the different IgG
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