F2756

Fluorescein di(β-D-galactopyranosid)

• Synonym(e): FDG
• Empirische Formel (Hill-System): C₃₂H₃₂O₁₅
• CAS-Nummer: 17817-20-8
• Molekulargewicht: 656.59
• MDL-Nummer: MFCD00077344
• UNSPSC-Code: 12352201
• PubChem Substanz-ID: 24894829
• NACRES: NC.07

Eigenschaften

• Assay: ≥98% (HPLC)
• Form: powder
• Löslichkeit: methanol: 20 mg/mL, clear, yellow
• Lagertemp.: −20°C
• SMILES String: OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C36OC(=O)c7ccccc67)c2)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1
• InChIKey: ZTOBILYWTYHOJB-WBCGDKOGSA-N

Beschreibung

Anwendung

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).

Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Biochem./physiol. Wirkung

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

Substrate

Fluorogenic substrate for β-galactosidase

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)