E8027

EphA2/Fc Chimera from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(e): Eck, Myk2, Sek
• MDL-Nummer: MFCD01867520
• UNSPSC-Code: 51111800

Eigenschaften

• Biologische Quelle: mouse
• Rekombinant: expressed in NSO cells
• Assay: >95% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: 0.078-5 ng/mL
• Mol-Gew.: monomer calculated mol wt 84 kDa; ~105 kDa by SDS-PAGE (reducing)
• Verpackung: pkg of 200 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Methode(n): ligand binding assay: suitable
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: Q03145
• Lagertemp.: −20°C
• Angaben zum Gen: mouse ... Epha2(13836)

Beschreibung

Allgemeine Beschreibung

EPHA2 (ephrin receptor A2) belongs to the Eph family of receptor tyrosine kinases. It is a type I transmembrane protein, and its exoplasmic domain consists of a ligand binding domain in its N-terminal, a cysteine-rich domain, and two fibronectin type III repeats. Its cytoplasmic domain contains a juxtamembrane region, a tyrosine kinase domain, a SAM (sterile-a-motif) motif, and a PDZ-binding domain.

Biochem./physiol. Wirkung

EPHA2 binds to the ephrin-A ligand and has diverse cellular function. EPHA2 has been shown to be an oncoprotein of importance in a range of cancers. EPHA2 is overexpressed in several human cancer types and promotes malignancy through a mechanism involving RhoA-dependent destabilization of adherens junctions. EPHA2 overexpression induces a FAK-dependent increase in MMP-2 expression and invasiveness and this process that can be reversed by ligation of EPHA2.

Member of the Eph receptor tyrosine kinase family shown to bind ephrin-A1, ephrin-A2, ephrin-A3, ephrin-A4, and ephrin-A5; involved in pattern formation and morphogenesis. EphA2 is expressed on endothelial cells and in tumor xenographs and may regulate tumor neovascularization.

Sonstige Hinweise

Extracellular domain of mouse EphA2 (amino acids 1-535) fused by means of a polypeptide linker to the Fc region of human IgG1 that is histidine-tagged at the C-terminus.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0.

Hinweis zur Analyse

The biological activity is measured by its ability to bind recombinant mouse ephrin-A1/Fc in an ELISA assay.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)