F5-5.F1

99072803, mouse spleen, Lymphoblastoid

• UNSPSC-Code: 41106514

Eigenschaften

• product name: F5-5.F1, 99072803
• Biologische Quelle: mouse spleen
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse Lymphocyte, friend leukemia

Beschreibung der Zelllinie

Investigation of differentiation-inducible leukaemia cells is considered important in the understanding of leukaemic or neoplastic state. Several ascites lines derived from friend virus-induced erythoid leukemia show an erythroid morphology and also, unusually high haem synthesis. Most cultured lines of friend virus-induced leukemia in mice, are known to be inducible to differentiate towards erythocytes. F-5-5.F1 is susceptible to the induction by both dimethyl and butyric acid.

Nährmedium

RPMI 1640 + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or

Sonstige Hinweise

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