JVM-13

96090516, human blood, Lymphoblastoid

• UNSPSC-Code: 41106514

Eigenschaften

• product name: JVM-13, 96090516
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: 46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian B-prolymphocytic leukaemia, EBV transformed

Beschreibung der Zelllinie

Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.

Anwendung

Study of B cell leukaemia

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18

Nährmedium

RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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