EMT6/AR10.0

96042326, mouse unknown/unspecified, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: EMT6/AR10.0, 96042326
• Biologische Quelle: mouse unknown/unspecified
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse mammary, drug-resistant

Beschreibung der Zelllinie

The drug resistant cell line EMT6/AR10.0 was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by continuous growth in increasing concentrations of doxorubicin. It was reported to show reduced intracellular drug accumulation and hyperexpression of P-glycoprotein. It is recommended to culture the cells without drug after resuscitation until the first passage.

Anwendung

Drug-resistance studies

Nährmedium

MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA)+ 10.0μg/ml doxorubicin + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5%CO₂; 37ºC; resuscitate in drug-free media, add doxorubicin after first passage

Sonstige Hinweise

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