3T3-MSV

90092701, mouse embryo, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: 3T3-MSV, 90092701
• Biologische Quelle: mouse embryo
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Growth factor
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

Product has lost contact inhibition

Ursprung der Zelllinie

Mouse Swiss 3T3 embryo, Moloney-MSV transformed

Beschreibung der Zelllinie

This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.

Anwendung

Cell growth studies

Nährmedium

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.

Sonstige Hinweise

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