M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: M-MSV-BALB/3T3, 90030802
• Biologische Quelle: mouse embryo
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 57
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

Beschreibung der Zelllinie

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

Anwendung

Transformation and virus studies: M-MSV, MuLV

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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