NB4 1A3

89121405, mouse nerve, Neuroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: NB4 1A3, 89121405
• Biologische Quelle: mouse nerve
• Wachstumsmodus: Adherent
• Karyotyp: Unstable
• Morphologie: Neuroblast
• Produkte: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C-1300 Neuroblastoma

Beschreibung der Zelllinie

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Anwendung

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Nährmedium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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