I-10

89031605, mouse testis, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: I-10, 89031605
• Biologische Quelle: mouse testis
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 40, hyperdiploid
• Morphologie: Epithelial
• Produkte: Steroids
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse BALB/c Leydig cell testicular tumour

Beschreibung der Zelllinie

Derived from a Leydig cell testicular tumour of a BALB/c mouse. The cells secrete steroids; this increases at saturation density. Secretion should be retained for over 50 passages. Exposure to 0.00001M cAMP stimulates secretion 4-5 fold. The major products are progesterone and its derivative 20 α-hydroxy-4-pregnen-3-one. It does not respond to interstitial cell stimulating hormone (ICSH) but is stimulated by cyclic adenosine monophosphate (AMP).

Anwendung

Tumourigenicity studies

Nährmedium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 6x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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