ZR-75-30

88113004, human breast, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: ZR-75-30, 88113004
• Biologische Quelle: human breast
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human breast carcinoma

Beschreibung der Zelllinie

Derived from ascitic fluid of a 47 year old woman with infiltrating ductal carcinoma. Ethnicity: Black.

Anwendung

Tumourigenicity studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 10,11
D16S539: 9
D5S818: 12,13
D7S820: 11,12
THO1: 8,9
TPOX: 10,11
vWA: 18,19

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Cells produce a significant amount of cellular debris appearing as black particles. This cell line grows relatively slowly and requires passaging approximately every 5 days.

Sonstige Hinweise

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