OX-39

86100905, Reactivity: Anti-CD25, rat

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myelom: NS0/1
• Reaktivität: Anti-CD25, rat
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse NS0/1 x Mouse BALB/c spleen

Beschreibung der Zelllinie

The antibody is directed against the rat IL-2 receptor found on T cell blasts and thymic dendritic cells. The OX-39 antigen has a molecular weight of 45-50kDa.

Immunogen

T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleens

Nährmedium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.