htr-20

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Human x Mouse Hybridoma
• Verpackung: tube of 5 μg 10080903-DNA-5UG; pkg of vial of cells 10080903-1VL
• ImmunoDon: Mouse (Mus musculus) spleen
• Myelom: PAI
• Reaktivität: Human CD120a (TNF receptor p55)
• Methode(n): cell culture | mammalian: suitable
• Isotyp: IgG2b
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Not specified

Immunogen

Human CD120a (TNF receptor p55)

Anwendung

Sandwich ELISA for soluble p55 TNF-R.

Nährmedium

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 25 U/ml Interleukin-6 (IL-6) + 5-10% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA maybe required to dislodge them from the plastic.

Subkultur-Routine

Seed cultures between 1-3 x 10⁵ cells/ml; Saturation density 8-10 x 10⁵ 5% CO₂; 37 °C.

Sonstige Hinweise

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