C7990

Anti-phospho-α-B Crystallin (54-64) (pSer⁵⁹) antibody produced in rabbit

affinity isolated antibody, lyophilized powder

• MDL-Nummer: MFCD06798481
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: lyophilized powder
• Speziesreaktivität: rat, bovine, human
• Methode(n): indirect immunofluorescence: 8 μg/mL using human brain from Alexander′s diseased patients; western blot: 0.5 μg/mL using recombinant bovine phospho-α-B crystallin (Ser59)
• UniProt-Hinterlegungsnummer: P02511
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: phosphorylation (pSer59)
• Angaben zum Gen: human ... CRYAB(1410) ; rat ... Cryab(25420)

Beschreibung

Allgemeine Beschreibung

α-B-crystallin (CRYAB) belongs to small heat-shock protein family with chaperone-like function. Almost entire lens proteins composed of crystallins and within that α-crystallin accounts for 40% of total lens protein. α-Crystallin is composed of two primary gene products, α-A and α-B. α-B crystallin in particular, has been detected in many tissues in the central nervous system, and is considered to be a useful marker in a variety of neurodegenerative diseases.

Spezifität

This antibody does not detect the unphosphorylated form of the protein.

Immunogen

synthetic phosphopeptide: FLRAPS(p)WIDTG

Anwendung

Anti-phospho-α-B Crystallin (54-64) (pSer⁵⁹) antibody is suitable for indirect immunofluorescence in 8μg/mL using human brain from Alexander′s diseased patients. It is also suitable for western blot analysis in concentration of 0.5μg/mL using recombinant bovine phospho-α-B crystallin (Ser⁵⁹).

Biochem./physiol. Wirkung

Anti-phospho-α-B Crystallin (54-64) (pSer⁵⁹) antibody is responsible for the optical properties of the lens and have been identified from metabolic enzymes and stress proteins. In many neurological diseases, α B-crystallin have found over-expressed and any alterations in this protein leads cataract and myopathy. In addition to maintaining proper refractive index, it also functions in a chaperone-like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of α-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance.

Physikalische Form

Lyophilized from phosphate buffered saline, pH 7.4, with 3% bovine serum albumin and 0.05% sodium azide.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids