C2273

Carboanhydrase aus Rindererythrocyten

For use as a marker in SDS-PAGE

• Synonym(e): Carbonat-Dehydratase, Carbonat-Hydro-Lyase
• CAS-Nummer: 9001-03-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-576-6
• MDL number: MFCD00081467
• EG-Nummer: 4.2.1.1 (BRENDA, IUBMB)
• Biological source: bovine erythrocytes

Eigenschaften

• biological source: bovine erythrocytes
• Quality Segment: 200
• form: powder
• mol wt: ~29 kDa
• packaging: vial of 5 mg
• storage temp.: 2-8°C

Beschreibung

General description

Carbonic Anhydrase is a metalloenzyme with zinc. The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).

Application

Carbonic Anhydrase has been used:

• as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samples
• as a molecular marker in gel filtration chromatography
• as a standard protein to test the recovery of CMA/20 microdialysis probe

Biochem/physiol Actions

Carbonic anhydrase is essential for the reversible hydration of carbon dioxide. It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways. Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)