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Merck

C-12921

Sigma-Aldrich

Human CD34+ Progenitor Cells (hCD34+-CB)

Isolated from cord blood, single donor, 100,000 cryopreserved cells

Synonym(e):

hCD34+-CB cells

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About This Item

UNSPSC-Code:
41106514
NACRES:
NA.81

Biologische Quelle

human umbilical cord blood

Verpackung

pkg of 100,000 cells

Morphologie

(progenitor)

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

dry ice

Lagertemp.

−196°C

Allgemeine Beschreibung

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Ursprung der Zelllinie

Cord Blood

Anwendung

CD34 is a glycosylated transmembrane protein and represents a well-known marker for primitive blood- and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial stem cells. Although the biological functions of CD34 are largely unknown, recent data suggest that CD34 is involved in maintenance of the progenitor cells in a phenotypically undifferentiated state. PromoCell offers CD34+ Progenitor Cells from the cord blood of healthy donors. The CD34+ Progenitor Cells contain two main cellular subpopulations, hematopoietic and endothelial progenitor cells. Therefore, CD34+ Progenitor Cells are suitable for a series of studies, e.g. directed differentiation into more committed types of blood cells and endothelial lineages.

Qualität

Rigid quality control tests are performed for each lot of CD34+ Progenitor Cells. They are routinely characterized by flow cytometry analyzing the viability, cell size, and the presence of significant markers, i.e. CD34. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warnhinweis

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subkultur-Routine

Click here for more information.

Sonstige Hinweise

Recommended Plating Density: 5000 cells per mlTested Markers: CD34 positive

Empfohlene Produkte

Recommended Primary Cell Culture Media:Link

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Tatjana Weidner et al.
Nature protocols, 16(7), 3210-3240 (2021-04-14)
Receptor targeting of vector particles is a key technology to enable cell type-specific in vivo gene delivery. For example, T cells in humanized mouse models can be modified by lentiviral vectors (LVs) targeted to human T-cell markers to enable them

Artikel

CD34+ Progenitor Cells are suitable for diverse studies, as well as for the directed differentiation into more committed blood cell phenotypes and endothelial lineages. 

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

Was sind Primärzellen? Ein umfassender Überblick über tierische und menschliche Primärzelllinien und -medien und deren Verwendung in biomedizinischen Zellkulturanwendungen. Primärkulturen sind im Vergleich zu Zelllinien weniger homogen.

Protokolle

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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