C-12566

Human Tracheal Smooth Muscle Cells (HTSMC)

500,000 proliferating cells

• UNSPSC-Code: 41106514
• NACRES: NA.81

Eigenschaften

• Biologische Quelle: human trachea
• Verpackung: pkg of 500,000 cells
• Morphologie: (smooth muscle)
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): asthma
• Versandbedingung: ambient
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

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Ursprung der Zelllinie

Trachea

Anwendung

Primary Human Tracheal Smooth Muscle Cells (HTSMC) are isolated from the trachea from single donors and are provided in a proliferating format. HTSMC stain positive for smooth muscle α-actin. They connect the endings of the tracheal cartilage by a contractile bridge called the paries membranaceus and consequently regulate the airway lumen. Under pathological conditions like asthma, the amount of smooth muscle cells in the trachea walls is increased leading to smooth muscle hypertrophy or hyperplasia. Hence, HTSMC are suitable for in vitro studies of asthma and other pulmonary diseases.

Qualität

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warnhinweis

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subkultur-Routine

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Sonstige Hinweise

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Empfohlene Produkte

Recommended Primary Cell Culture Media:Link

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable