A9292

Anti-Horse IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(e): Rabbit Anti-Horse IgG (whole molecule)−HRP
• MDL-Nummer: MFCD00162385
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: peroxidase conjugate
• Antikörperform: IgG fraction of antiserum
• Antikörper-Produkttyp: secondary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Methode(n): direct ELISA: 1:15,000; dot blot: 1:100,000-1:200,000 (chemiluminescent); immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-horse IgG (whole molecule)−peroxidase antibody is specific for IgG in horses.

Immunogen

Horse IgG

Anwendung

Anti-horse IgG (whole molecule)−peroxidase antibody may be used in dot blot (1:8,000-1:10,000), immunohistochemistry (formalin-fixed, paraffin-embedded sections, at 1:150 dilution) and direct ELISA (1:15,000).

The level of S2-specific antibodies in test samples of horse serum was determined by ELISA using HRP-conjugated rabbit anti-horse IgG at a 1:35000 dilution with incubation for 45 minutes at room temperature.

Biochem./physiol. Wirkung

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (antigen-binding fragments) fragments or bivalent F(ab′)2 fragments.  These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

Physikalische Form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Angaben zur Herstellung

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H317,H334
• P-Sätze: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Gefahreneinstufungen: Resp. Sens. 1 - Skin Sens. 1
• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable