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A5295

Sigma-Aldrich

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Rabbit Anti-Bovine IgG (whole molecule)−HRP

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About This Item

MDL-Nummer:
MFCD00162171
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Konjugat

peroxidase conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

bovine

Methode(n)

direct ELISA: 1:20,000
dot blot: 1:80,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Verwandte Kategorien

Allgemeine Beschreibung

IgG (immunoglobulin G) contributes to 10-20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

purified bovine IgG

Anwendung

Anti-Bovine IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • bovine viral diarrhea virus (BVDV) plaque
  • focus-forming assays

Biochem./physiol. Wirkung

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physikalische Form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Angaben zur Herstellung

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Infectious bovine viral diarrhea virus (strain NADL) RNA from stable cDNA clones: a cellular insert determines NS3 production and viral cytopathogenicity
Mendez E, et al.
Journal of virology, 72(6), 4737-4745 (1998)
Rafaella P M Guimarães-Peixoto et al.
PLoS neglected tropical diseases, 12(4), e0006371-e0006371 (2018-04-13)
Bovine cysticercosis is a worldwide distributed zoonosis caused by the larval form of Taenia saginata present in bovine muscles. The diagnosis is based on the postmortem inspection at slaughterhouses and consists of the macroscopic visualization of lesions caused by cysticercosis
Davita Pillay et al.
PloS one, 8(10), e78565-e78565 (2013-11-10)
Diagnosis of African animal trypanosomosis is vital to controlling this severe disease which hampers development across 10 million km(2) of Africa endemic to tsetse flies. Diagnosis at the point of treatment is currently dependent on parasite detection which is unreliable
Karukriti Kaushik Ghosh et al.
Applied and environmental microbiology, 84(6) (2017-12-23)
In this study, the effect of the host stress hormone catecholamine on Leptospira gene transcripts encoding outer membrane proteins was investigated. There was no impact of catecholamine supplementation on the in vitro growth pattern of Leptospira interrogans; however, 7 genes
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