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Merck

A3861

Sigma-Aldrich

Anti-Palladin (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(e):

Anti-PALLD

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~140 kDa (another band at 90 kDa)

Speziesreaktivität

human

Verpackung

antibody small pack of 25 μL

Methode(n)

immunoprecipitation (IP): 10-20 μL using HeLa cell lysates
indirect immunofluorescence: 1:250-1:500 using human HeLa cells
western blot: 1:250-1:500 using whole extracts of human HeLa cells

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PALLD(23022)
mouse ... Palld(72333)

Allgemeine Beschreibung

Anti-Palladin (C-terminal) is produced in rabbit using as the immunogen a synthetic peptide corresponding to a sequence at the C-terminal of human palladin, conjugated to KLH. Palladin, an actin associated Ig-domain protein is mapped to human chromosome 4q32. This phosphoprotein is widely expressed in vertebrate cells and tissues. Palladin is expressed as three major isoforms of 90, 140 and 200 kDa.

Anwendung

Anti-Palladin (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Rabbit anti-Palladin (C-terminal) antibody can be used for immunoprecipitation (10-20μL), indirect immunofluorescence (1:250-1:500) and western blot (1:250 -1:500) using HeLa cells.

Biochem./physiol. Wirkung

Palladin is a component of actin-containing microfilaments that control cell shape, adhesion, and contraction. Its expression is required for normal mammalian embryogenesis since inactivation of paladin leads to embryonic lethality. Palladin interacts with the actin-associated proteins -actinin, VASP, ezrin, and profilin, suggesting that it is required for normal actin cytoskeleton organization. It serves as a transcriptional regulator, which controls the expression of smooth muscle cell genes.
Palladin is a microfilament-associated phosphoprotein that is known to colocalize with alpha-actinin at cell-cell junctions, stress fibres, embryonic Z-lines, and focal adhesions. Palladin abnormalities have been linked to familial pancreatic cancer. Rabbit anti-Palladin (C-terminal) antibody binds to human palladin. Detection of the palladin band by immunoblotting is specifically inhibited by the immunizing peptide. A nonspecific band of approx. 55kDa may be detected in some cell extract preparations.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Meta-analysis of sex differences in gene expression in schizophrenia
BMC Systems Biology, 10(1), S9-S9 (2016)
Palladin mediates stiffness-induced fibroblast activation in the tumor microenvironment
McLane JS and Ligon LA
Biophysical Journal, 109(2), 249-264 (2015)
The actin associated protein palladin is important for the early smooth muscle cell differentiation
Jin L, et al.
PLoS ONE, 5(9), e12823-e12823 (2010)
Kay L Pogue-Geile et al.
PLoS medicine, 3(12), e516-e516 (2006-12-30)
Pancreatic cancer is a deadly disease. Discovery of the mutated genes that cause the inherited form(s) of the disease may shed light on the mechanism(s) of oncogenesis. Previously we isolated a susceptibility locus for familial pancreatic cancer to chromosome location
Characterization of human palladin, a microfilament-associated protein
Mykka?nen OM, et al.
Molecular Biology of the Cell, 12(10), 3060-3073 (2001)

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