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Merck

A3435

Sigma-Aldrich

D-Alanin–Agarose

saline suspension

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About This Item

MDL-Nummer:
UNSPSC-Code:
23151817
NACRES:
NA.56

Form

saline suspension

Kennzeichnungsgrad

2-8 μmol per mL

Matrix

cross-linked 4% beaded agarose

Matrixaktivierung

cyanogen bromide

Matrixanbindung

amino

Matrix-Spacer

1 atom

Lagertemp.

2-8°C

Anwendung

D-Alanine Agarose can be used in proteomics and protein chromatography. It has been utilized in experiments with antibiotics of the vancomycin group to determine the role of micellular aggregates in the high binding affinity of teicoplanin to D-alanyl-D-alanine-agarose.

Physikalische Form

Suspension in 2.0 M NaCl, enthält 0.02% Thiomersal

Analysenzertifikate (COA)

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A Corti et al.
Clinical chemistry, 31(10), 1606-1610 (1985-10-01)
A solid-phase enzyme-receptor assay (SPERA) has been developed for glycopeptide antibiotics of the vancomycin class such as teicoplanin, vancomycin, ristocetin, avoparcin, actaplanin, A-47934, A-41030, and A-35512-B. The assay exploits the mechanism of most action of these antibiotics, which is based
A Corti et al.
Journal of applied biochemistry, 7(2), 133-137 (1985-04-01)
Teicoplanin, as well as the other antibiotics of the vancomycin group, was shown to bind specifically to D-alanyl-D-alanine-agarose (D-Ala-D-Ala-AGA) (A. Corti and G. Cassani, Appl. Biochem. Biotechnol. 11, 101-110 (1985)). This finding is extended, showing that the binding is as
Catherine F Silverio et al.
Electrophoresis, 24(5), 808-815 (2003-03-11)
Binding constants between the glycopeptides teicoplanin (Teic) and ristocetin (Rist) and their derivatives to D-Ala-D-Ala terminus peptides were determined by on-column receptor synthesis coupled to partial-filling affinity capillary electrophoresis (PFACE) or affinity capillary electrophoresis (ACE). In these techniques, the column
Makoto Umeda et al.
Biochemical and biophysical research communications, 466(4), 717-722 (2015-09-09)
Arginine, a semi-essential amino acid, is known as one of the most strongest insulin secretagogues in a glucose-dependent manner, but major mechanism is unknown. Arginine induced insulin secretion in mice as well as β cell line, NIT-1, in which more

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