A2668
Anti-Actin antibody produced in rabbit
whole antiserum
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About This Item
MDL-Nummer:
UNSPSC-Code:
12352203
Empfohlene Produkte
Biologische Quelle
rabbit
Konjugat
unconjugated
Antikörperform
whole antiserum
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Enthält
15 mM sodium azide
Speziesreaktivität
mouse, rat, human, chicken
Methode(n)
indirect immunofluorescence: 1:40 using cultured chicken fibroblasts
western blot: suitable using rabbit skeletal muscle α-actin1
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−20°C
Angaben zum Gen
human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
Allgemeine Beschreibung
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the func tional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). Difficulties have been encountered in producing polyclonal antibodies due to the highly conserved nature of actin.
Rabbit Anti-Actin reacts with pure actin by counter immunoelectrophoresis. The product also stains the I-bands of stretched skeletal muscle.
Rabbit polyclonal anti-Actin antibody reacts with rat, chicken, human, and mouse actin. By immunoblotting, cross-reactivity with other proteins may be observed.
Immunogen
actin from chicken back muscle
Anwendung
May be used for studying the intracellular distribution of actin structures and their functions in the contractile system.
Rabbit anti-actin antibody has been used for western blot assays using mouse lung homogenates and human umbilical artery endothelial cell (HUAEC) lysates.
Rabbit anti-actin antibody was used to visualize actin in human sperm smears. Samples were incubated with the antibody (1:80) for 2 hours at 37 degrees.
Fluorescent microscopy was performed on bovine fallopian tube epithilial cells to visualize actin aggregates following inoculation with c. parvum sporozites using the rabbit anti-actin antibody from Sigma.
Fluorescent microscopy was performed on bovine fallopian tube epithilial cells to visualize actin aggregates following inoculation with c. parvum sporozites using the rabbit anti-actin antibody from Sigma.
Rabbit polyclonal anti-Actin antibody is used to tag actin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of actin in stress fibers and I-bands of skeletal muscle cells.
Physikalische Form
Rabbit Anti-Actin is supplied as a liquid containing 0.1% sodium azide as preservative.
Sonstige Hinweise
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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