A2264

β-N-Acetylglucosaminidase aus Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥8 units/mg protein

• Synonym(e): β-N-Acetyl-D-hexosaminid-N-acetylhexosaminohydrolase aus Schwertbohnen, β-N-Acetylhexosaminidase
• CAS-Nummer: 9012-33-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-730-2
• MDL number: MFCD00081402
• EG-Nummer: 3.2.1.52 (BRENDA, IUBMB)
• Specific activity: ≥8 units/mg protein
• Biological source: Canavalia ensiformis

Eigenschaften

• biological source: Canavalia ensiformis
• Quality Segment: 200
• form: ammonium sulfate suspension
• specific activity: ≥8 units/mg protein
• foreign activity: α- and β-galactosidase, and α-L-fucosidase ≤0.1%, α-mannosidase ≤0.5%
• storage temp.: 2-8°C

Beschreibung

Application

β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer′s Disease [1]. Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms [2].

Biochem/physiol Actions

Wird auch als β-N-Acetylhexosaminidase bezeichnet. Es wurde die Freisetzung von terminalem β-verknüpften N-Acetylglucosamin und N-Acetylgalactosamin durch dieses Enzym beobachtet.

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.

Physical form

Suspension in 2.5 M (NH₄)₂SO₄, pH 7.0

Analysis Note

Bei pH 4.0 wird p-Nitrophenyl β-N-acetylgalactosaminid mit ca. 50% der Hydrolysegeschwindigkeit von p-Nitrophenyl β-N-acetylglucosaminid bei pH 5.0 hydrolysiert.

Other Notes

1 U hydrolysiert 1.0 μmol p-Nitrophenyl N-acetyl-β-D-glucosaminid zu p-Nitrophenol und N-acetyl-D-glucosamin pro Minute bei pH 5.0 und 25 °C.

Sicherheitshinweise

• flash_point_f: Not applicable
• flash_point_c: Not applicable
• Lagerklasse: 10 - Combustible liquids