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Merck

A0793

Sigma-Aldrich

Anti-Dog IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(e):

Rabbit Anti-Dog IgG (whole molecule)–AP

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

alkaline phosphatase conjugate

Antikörperform

IgG fraction of antiserum

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

dog

Methode(n)

direct ELISA: 1:6,000
dot blot: 1:4,000
western blot (chemiluminescent): 1:4,000

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

An immunoglobulin has two heavy chain and two light chain connected by disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. In dogs four subclasses of IgG (IgG1 -IgG4) are present.
Dog IgGs are glycoprotein antibodies that regulate several immunological responses. Canine IgGs can be used as markers for detecting immune-mediated haemolytic anaemia, adverse food reactions and also for assessing the risk of L. infantum transmission in dogs.

Spezifität

Anti-Dog IgG (whole molecule) antibody is specific for IgG in dogs when tested against normal dog serum and dog IgG by double-diffusion and immunoelectrophoretic assays prior to conjugation.

Immunogen

IgG isolated from pooled normal dog serum

Anwendung

Anti-Dog IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA), and immunoblotting.

Biochem./physiol. Wirkung

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physikalische Form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Delivery of recombinant gene product to canine brain with the use of microencapsulation
Barsoum SC, et al.
The Journal of Laboratory and Clinical Medicine, 142(6), 399-413 (2003)
Comparative evaluation of several methods for Canine Visceral Leishmaniasis diagnosis
Silva DT da, et al.
Revista Brasileira de Parasitologia veterinaria = Brazilian Journal of Veterinary Parasitology : Org?o Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 23(2), 179-186 (2014)
C Sriwichai et al.
The Journal of small animal practice, 52(9), 476-483 (2011-09-08)
Canine allo- or autoantibodies are clinically important, but attachment of these immunoglobulin G (IgG) antibodies does not produce observable haemagglutination. Antibody to canine globulins is required to demonstrate sensitisation of red blood cells. Commercial reagents are available, but these often
Michaela Vlkova et al.
PLoS neglected tropical diseases, 5(10), e1344-e1344 (2011-10-25)
Phlebotomine sand flies are blood-sucking insects that can transmit Leishmania parasites. Hosts bitten by sand flies develop an immune response against sand fly salivary antigens. Specific anti-saliva IgG indicate the exposure to the vector and may also help to estimate
Joseph A Impellizeri et al.
Journal of translational medicine, 16(1), 349-349 (2018-12-13)
we have recently shown that Tel-eVax, a genetic vaccine targeting dog telomerase (dTERT) and based on Adenovirus (Ad)/DNA Electro-Gene-Transfer (DNA-EGT) technology can induce strong immune response and increase overall survival (OS) of dogs affected by multicentric Diffuse Large B cell

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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