G1N9604

GenElute^™ 96 Well Tissue Genomic DNA Purification Kit

4 x 96 well plate, sufficient for 4 96-well plate purifications

• Synonym(e): High Throughput Purification, Tissue Genomic DNA
• UNSPSC-Code: 12352200
• NACRES: NA.55

Eigenschaften

• Verwendung: sufficient for 4 96-well plate purifications
• Qualität: 4 x 96 well plate
• Lagertemp.: 15-25°C

Beschreibung

Allgemeine Beschreibung

GenElute 96 Well Tissue Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations and hazardous compounds such as phenol and chloroform.

GenElute 96 Well Tissue Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations, and hazardous compounds such as phenol and chloroform.

The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.

The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.

Anwendung

The purified genomic DNA is ready for use in downstream applications such as:

• PCR
• Southern blots
• cloning
• restriction digest
• enzymatic reactions

Leistungsmerkmale und Vorteile

• Starting material: ≤ 20 mg tissue total (mouse, rat or whole sample) or ≤ 1 X 10⁶ cultured cells or bacteria per well
• Average Expected yield: 10 - 20 μg/well (bacteria: 1.5 - 2.5 μg/well)
• Elution volume: 100 μl
• Time required: 70 min/plate
• Innovative wash plate minimizes risk of cross-contamination
• Processing possible under vacuum or centrifugation
• Suitable for manual and automated processing

Prinzip

The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol, which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane, several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.

Rechtliche Hinweise

GenElute is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Piktogramme: GHS02,GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H226,H302,H315,H319,H334,H335,H336
• P-Sätze: P210 - P233 - P240 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Central nervous system, Respiratory system
• Lagerklassenschlüssel: 3 - Flammable liquids
• Flammpunkt (°F): 75.2 °F
• Flammpunkt (°C): 24 °C