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Pyroglutamataminopeptidase aus Pyrococcus furiosus, rekombinant aus E. coli
7-13 mU (per vial)
Synonym(e):
Pyrase, Pyroglutamyl-Peptidase I, Pyrrolidon-Carboxyl-Peptidase
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About This Item
Empfohlene Produkte
Rekombinant
expressed in E. coli
Form
lyophilized
Spezifische Aktivität
7-13 mU (per vial)
Mol-Gew.
Mr ~28000
Lagertemp.
−20°C
Verwandte Kategorien
Verpackung
Packung mit 0.01 Unit
Einheitendefinition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol Pyroglutamate-p-nitroanilide per minute at pH 7.0 and 37 °C
Hinweis zur Analyse
Enzym Aktivität: die opimale Temperatur ist 95-100° (stabil bis zu 75°C), der optimale pH ist 6-9 (stabil von pH 6-9); Inhibitoren: PCMB, Hg+
Sonstige Hinweise
An enzyme that removes pyroglutamic acid (pGlu) from pGlu-peptide and proteins; employed in Edman degradation.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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J Mozdzanowski et al.
Analytical biochemistry, 260(2), 183-187 (1998-07-11)
For larger proteins, efficient deblocking prior to Edman sequencing is especially important to obtain quality, extended sequencing data which is limited by the stepwise accumulation of background from the random acid hydrolysis of the protein. Therefore, any portion that remains
Y Shimada et al.
Journal of biochemistry, 106(3), 383-388 (1989-09-01)
The cDNA clone of Geotrichum candidum (Geo.) lipase was isolated from the Geo. cDNA library by colony hybridization using 32P-labeled oligonucleotides corresponding to a partial amino acid sequence of this enzyme. The nucleotide sequence of the cDNA determined by the
Jai K Kaushik et al.
Biochemistry, 45(23), 7100-7112 (2006-06-07)
Pyrrolidone carboxyl peptidases (PCPs) from hyperthermophiles have a structurally conserved and completely buried Glu192 in the hydrophobic core; in contrast, the corresponding residue in the mesophile protein is a hydrophobic residue, Ile. Does the buried ionizable residue contribute to stabilization
Adolfo Varona et al.
American journal of physiology. Renal physiology, 292(2), F780-F788 (2006-09-21)
Peptides play important roles in cell regulation and signaling in many tissues and are regulated by peptidases, most of which are highly expressed in the kidney. Several peptide convertases have a function in different tumor stages, and some have been
Noriko Higaki-Sato et al.
Journal of agricultural and food chemistry, 54(19), 6984-6988 (2006-09-14)
In order to determine pyroglutamic acid levels in plasma, we developed a method based on precolumn derivatization of the carboxyl group of pyroglutamic acid with 2-nitrophenylhydrazine. Eight-week-old male SD strain rats were administered 200 mg of an acidic peptide fraction
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