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N-[4-(2-Benzimidazolyl)-phenyl]-maleinimid

Name: <I>N</I>-[4-(2-Benzimidazolyl)-phenyl]-maleinimid
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Synonym(e):

BIPM

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About This Item

Empirische Formel (Hill-System):

C₁₇H₁₁N₃O₂

CAS-Nummer:

27030-97-3

Molekulargewicht:

289.29

Beilstein:

755407

EG-Nummer:

248-173-3

MDL-Nummer:

MFCD00042767

UNSPSC-Code:

12352200

PubChem Substanz-ID:

57646978

Produktlinie

BioReagent

Assay

≥98.0% (HPLC)

Form

solid

Löslichkeit

H₂O: soluble
acetone: soluble

Fluoreszenz

λ_ex 315 nm; λ_em 365 nm (after derivatization with 2-mercaptoethanol)

Eignung

suitable for fluorescence

SMILES String

O=C1C=CC(=O)N1c2ccc(cc2)-c3nc4ccccc4[nH]3

InChI

1S/C17H11N3O2/c21-15-9-10-16(22)20(15)12-7-5-11(6-8-12)17-18-13-3-1-2-4-14(13)19-17/h1-10H,(H,18,19)

InChIKey

NZDOXVCRXDAVII-UHFFFAOYSA-N

Sonstige Hinweise

Fluoreszenz-Sonde in Proteinen, für Microfluorometrie von Thiolverbindungen verwendet (Nachweisgrenze: 10^-6 m Thiol Konz.); Review

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

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Stopped flow measurement of conformational change induced by phosphorylation in (Na+,K+)-ATPase modified with N-[p-(2-benzimidazolyl)phenyl]maleimide.

K Taniguchi et al.

The Journal of biological chemistry, 258(11), 6927-6931 (1983-06-10)

The addition of ATP to (Na+,K+)-ATPase (EC 3.6.1.3) of pig kidney modified with N-[p-(2-benzimidazolyl)phenyl]maleimide induced three different modes of fluorescence change: positive, biphasic, and negative, which were observed, respectively, in the presence of Mg2+ with 16 or 160, 320 or

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Conformational change accompanying formation of oligomycin-induced Na(+)-bound forms and their conversion to ADP-sensitive phosphoenzymes in Na+,K(+)-ATPase.

K Taniguchi et al.

Journal of biochemistry, 109(2), 299-306 (1991-02-01)

Oligomycin reduced the fluorescence intensity of an N-(p-(2-benzimidazoly)phenyl) maleimide (BIPM) probe at Cys-964 of the alpha-chain of pig kidney Na+,K(+)-ATPase with increase in the concentration of Na+ with a Hill coefficient of nh = 0.77 with Kh = 231 mM.

Fluorescent thiol reagents. V. Microfluorometry of thiol compounds with a fluorescent-labeled maleimide.

T Sekine et al.

Analytical biochemistry, 48(2), 557-568 (1972-08-01)

Photo-cross-linking of rabbit skeletal troponin I deletion mutants with troponin C and its thiol mutants: the inhibitory region enhances binding of troponin I fragments to troponin C.

P K Jha et al.

Biochemistry, 35(34), 11026-11035 (1996-08-27)

Contraction of vertebrate striated muscle is regulated by the strong Ca(2+)-dependent interaction between troponin I (TnI) and troponin C (TnC). To critically evaluate this interaction, we generated four recombinant deletion fragments of rabbit fast skeletal TnI: the NH2-terminal fragment (TnI1-94)

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