PPB023

Tris buffer

Pre-set, pH 8.1, pHast Pack^™, powder

• Synonym(e): 0.1M Tris
• UNSPSC-Code: 12161700
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100
• Produktlinie: pHast Pack^™
• Form: powder
• Lagerbedingungen: (Tightly closed. Dry)
• Verunreinigungen: ≤5 ppm Heavy Metals (lead)
• Farbe: white
• pH-Wert: 8.1
• Kationenspuren: Fe: ≤10 ppm
• Fremdaktivität: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Anwendung

Intended for use in:

• Cell culture (sterile filter recommended)
• Western blot
• Protein electrophoresis
• DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

Leistungsmerkmale und Vorteile

• Ready-to-use for quick buffer prep
• No measuring and adjusting pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Verpackung

Foil pouches

Rekonstituierung

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

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Rechtliche Hinweise

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1