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Merck

46640

Supelco

7-Fluorbenzofurazan-4-sulfonsäure Ammoniumsalz

for HPLC derivatization, LiChropur, ≥98.5% (HPLC)

Synonym(e):

4-Fluor-7-sulfobenzofurazan Ammoniumsalz, 7-Fluorbenz-2,1,3-oxadiazol-4-sulfonsäure Ammoniumsalz, Ammonium-7-fluor-benzofurazan-4-sulfonat, SBD-F

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About This Item

Lineare Formel:
C6H3FN2O4S · NH3
CAS-Nummer:
Molekulargewicht:
235.19
Beilstein:
5199564
MDL-Nummer:
UNSPSC-Code:
12000000
PubChem Substanz-ID:
NACRES:
NA.22

Qualität

for HPLC derivatization
LiChropur

Qualitätsniveau

Assay

≥98.5% (HPLC)

Methode(n)

HPLC: suitable

Fluoreszenz

λex 385 nm; λem 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione)

Lagertemp.

2-8°C

SMILES String

N.OS(=O)(=O)c1ccc(F)c2nonc12

InChI

1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3

InChIKey

JXLHNMVSKXFWAO-UHFFFAOYSA-N

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Anwendung

Fluoreszierende Sonde für Thiole und die Vorsäulen-Markierung von biologischen Thiolen für die HPLC.

Verpackung

Bodenlose Glasflasche. Der Inhalt befindet sich innerhalb des eingeschobenen Schmelzkegels.

Empfohlene Produkte

LiChropur-Reagenzien eignen sich ideal für die HPLC- oder LC-MS-Analyse

Rechtliche Hinweise

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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B L Ling et al.
Journal of pharmaceutical and biomedical analysis, 10(10-12), 985-988 (1992-10-01)
The present investigation analyses the potentials of capillary chromatography using packed fused silica capillaries filled with 5 microns RP-18 for the fluorescence determination of glutathione in human blood samples. Adaptation of conventional HPLC equipment for miniaturized chromatographic assays proved successful.
Saori Ichinose et al.
Biomedical chromatography : BMC, 23(9), 935-939 (2009-04-09)
A semi-micro column HPLC-fluorescence method for routine determination of thiol derivatives such as homocysteine (Hcy), cysteine (Cys) and cysteamine (CA) is described. The thiol derivatives labeled with ammonium-7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F) were isocratically separated within 12 min on a semi-micro ODS column
N P Dudman et al.
Clinical chemistry, 42(12), 2028-2032 (1996-12-01)
The recognition of homocysteine as a vascular risk factor has led to increased clinical interest in assaying plasma homocysteine concentrations. Our aim was to improve the reliability of a widely used assay based on HPLC of the fluorescent 7-benzo-2-oxa-1, 3-diazole-4-sulfonic
Lila Otani et al.
Bioscience, biotechnology, and biochemistry, 75(11), 2119-2124 (2011-11-08)
The analytical method was optimized for L-cysteine (Cys) in rat plasma with co-existing L-cystine (Cyss). We observed that more than 100% Cyss in rat plasma was converted to Cys under typical conditions for the conversion with 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). Another conversion
I Daskalakis et al.
Biomedical chromatography : BMC, 10(5), 205-212 (1996-09-01)
A sensitive HPLC-fluorescence method for determining total endogenous plasma homocysteine (Hcy), cysteine (Cys) and cysteinylglycine (Cys-Gly) following derivatization with ammonium 7-fluoro 2,1,3-benzoxadiazole-4-sulphonate (SBD-F) is described. Quantitation utilizes an internal standard, 2-mercaptoethylamine. The derivatization procedure has been optimized for concentration of

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