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11427857910

Roche

Fluorescein-12-UTP

≥85% (HPLC), solution

Synonym(e):

Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)

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About This Item

Empirische Formel (Hill-System):
C39H41N4O22P3
CAS-Nummer:
134367-01-4
Molekulargewicht:
1010.68
UNSPSC-Code:
41116100

Qualitätsniveau

100

Assay

≥85% (HPLC)

Form

solution

Mol-Gew.

1034.4

Verpackung

pkg of 25 μL (250 nmol; 10 mM)

Hersteller/Markenname

Roche

Lagertemp.

−20°C

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Allgemeine Beschreibung

Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C39H37N4O22P3Li4.

Anwendung

Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.
For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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