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Wichtige Dokumente
11426320001
Roche
Anti-Fluorescein
from mouse IgG1 (clone B13-DE1)
Synonym(e):
antibody
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352200
Empfohlene Produkte
Biologische Quelle
mouse
Konjugat
unconjugated
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
B13-DE1, monoclonal
Form
lyophilized
Verpackung
pkg of 100 μg
Hersteller/Markenname
Roche
Isotyp
IgG1
Lagertemp.
2-8°C
Allgemeine Beschreibung
Monoclonal antibody to fluorescein.
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.
The antibody does not contain any protein and can hence be used for coating and labeling.
Contents
Lyophilizate, stabilized
Spezifität
The monoclonal antibody reacts with free and bound fluorescein.
Anwendung
Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Angaben zur Herstellung
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: Phosphate buffered saline, pH 7.4
Using water instead the antibody can precipitate.
Rekonstituierung
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Reconstitute for 15 minutes.
Reconstitute for 15 minutes.
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
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Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Aquatic Chronic 3 - Skin Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 2
Flammpunkt (°F)
does not flash
Flammpunkt (°C)
does not flash
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