PS1089

PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb

liquid, Calbiochem^®

• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: liquid
• Enthält: ≤0.1% sodium azide as preservative
• Speziesreaktivität: human
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Isotyp: IgG
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: phosphorylation (pSer188)
• Angaben zum Gen: human ... RHOA(387)

Beschreibung

Allgemeine Beschreibung

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~22 kDa RhoA protein when expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

Recognizes the ~22 kDa RhoA protein, expressed as a GST fusion protein (total ~49 kDa) and phosphorylated in vitro by PKA.

This PhosphoDetect Anti-RhoA (pSer¹⁸⁸) Rabbit pAb is validated for use in Immunoblotting, ELISA for the detection of RhoA (pSer¹⁸⁸).

Immunogen

Human

a synthetic peptide corresponding to amino acids surrounding the Ser¹⁸⁸ phosphorylation site of human RhoA, coupled to KLH

Anwendung

Immunoblotting (1:1000)
ELISA (1:2000)

Verpackung

Please refer to vial label for lot-specific concentration.

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In PBS, 1 mg/ml BSA, 50% glycerol.

Rekonstituierung

Do not aliquot.

Hinweis zur Analyse

Positive Control
RhoA GST fusion protein

Sonstige Hinweise

Does not recognize unphosphorylated RhoA. The immunogen sequence is conserved in rat and mouse RhoA, but cross-reactivity has not been tested. The immunogen sequence is not found in the RhoB and RhoC sequences. For immunoblotting, the membrane was incubated with antibody diluted in PBS/5% nonfat milk/0.04% Tween^® 20 detergent for 1 h at room temperature. Antibody should be titrated for optimal results in individual systems.

Ellerbroek, S.M., et al. 2003. J. Biol. Chem.278, 19023.
Sawada, N., et al. 2001. Biochem. Biophys. Res. Commun. 280, 798.
Dong, J.M., et al. 1998. J. Biol. Chem.273, 22554.
Lang, P., et al. 1996. EMBO J.15, 510.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1