MABT165

Anti-p130cas/BCAR1 Antibody, clone 2C1.1

clone 2C1.1, from mouse

• Synonym(e): Breast cancer anti-estrogen resistance protein 1, Cas scaffolding protein family member 1, Crk-associated substrate, p130Cas
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 2C1.1, monoclonal
• Speziesreaktivität: human
• Methode(n): western blot: suitable
• Isotyp: IgMκ
• NCBI-Hinterlegungsnummer: NP_055382
• UniProt-Hinterlegungsnummer: P56945
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... BCAR1(9564)

Beschreibung

Allgemeine Beschreibung

Breast cancer anti-estrogen resistance protein 1 (UniProt P56945; also known as Cas scaffolding protein family member 1, Crk-associated substrate, p130Cas) is encoded by the BCAR1 (also known as CAS, CAS1, CASS1, CRKAS) gene (Gene ID 9564) in human. p130Cas/BCAR1 is an adaptor protein that belongs to the CAS family of scaffold proteins, members of which also include Nedd9, HEF-1 (CAs-L), EFS, and CASS4. Structurally, p130Cas is composed of a Src-homology 3 (SH3) domain, an adjacent large substrate-binding domain containing 15 repetitions of the YxxP motif, a main site of tyrosine phosphorylation on the p130Cas/BCAR1 molecule that generates SH2-binding sites, a proline and serine rich region, and a highly conserved four-helix bundle (focal adhesion targeting [FAT] domain). p130Cas/BCAR1 plays an important role in transducing signaling originated from mechanical stimuli, extracellular matrix (ECM) adhesion, as well as growth factor stimulation cascades during normal development and diseases.

Spezifität

Expected to react with all eight spliced isoforms of human p130Cas reported by UniProt (P56945-1 through P56945-8).

Immunogen

GST-tagged recombinant human p130cas/BCAR1 C-terminal fragment.

Qualität

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected p130cas/BCAR1 in 10 µg of A431 cell lysate.

Zielbeschreibung

~93 kDa observed. 93.14-97.88 kDa (isoforms 1-3 and isoforms 5-8) and 77.73 kDa (isoform 4) calculated. Fully phosphorylated target band migrates with an apparent molecular size of ~130 kDa

Physikalische Form

Format: Purified

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2