MABS1145

Anti-3-PGDH Antibody, clone TK-08 13A8

clone TK-08 13A8, from mouse

• Synonym(e): D-3-phosphoglycerate dehydrogenase, 3-PGDH
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: TK-08 13A8, monoclonal
• Speziesreaktivität: human
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_006614
• UniProt-Hinterlegungsnummer: O43175
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PHGDH(26227)

Beschreibung

Allgemeine Beschreibung

D-3-phosphoglycerate dehydrogenase (EC1.1.1.95; UniProt O43175; also known as 3-PGDH, Epididymis secretory protein Li 113) is encoded by the PHGDH (also known as 3PGDH, HEL-S-113, NLS, NLS1, PGAD, PGDH, PDG, PGD, PGDH3, PHGDHD, SERA) gene (Gene ID 26227) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. L-serine is an important precursor for macromolecule biosynthesis. 3-PGDH catalyzes the first and rate-limiting step of the serine synthesis pathway from 3-phosphoglycerate to phospho-hydroxypyruvate and is important during tumor development. The PHGDH gene is frequently amplified and the enzyme is overexpressed in 70% of the estrogen receptor-negative breast cancers, 39% of melanoma, and cervical cancers.

Immunogen

Recombinant protein corresponding to human 3-PGDH.

Anwendung

Detect PHGDH using this mouse Monoclonal Anti-3-PGDH Antibody, clone TK-08 13A8, Cat. No. MABS1145, validated for use in Western Blotting.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected 3-PGDH in 10 µg HEK293 and Raji cell lysates.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected 3-PGDH in human salivary gland tissue sections.

Qualität

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 3-PGDH in 10 µg of HeLa cell lysate.

Zielbeschreibung

~57 kDa observed

Physikalische Form

Format: Purified

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1