MABN609

Anti-OCRL/INPP5b Antibody, clone N166A/26

clone N166A/26, from mouse

• Synonym(e): Inositol polyphosphate 5-phosphatase OCRL-1, Lowe oculocerebrorenal syndrome protein
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: N166A/26, monoclonal
• Speziesreaktivität: human, rat
• Methode(n): immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_000267
• UniProt-Hinterlegungsnummer: Q01968
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... OCRL(4952)

Beschreibung

Allgemeine Beschreibung

Inositol polyphosphate 5-phosphatase OCRL-1 (ORCL) is also called Lowe oculocerebrorenal syndrome protein. ORCL is a phosphotase that converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate, as well as inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate. ORCL is involved in primary cilia assembly and may also have a role in lysosomal membrane trafficking. ORCL is expressed in brain, skeletal muscle, heart, kidney, lung, placenta, fibroblasts, retina and the retinal pigment epithelium. OCRL defects can cause Lowe oculocerebrorenal syndrome (OCRL) and Dent disease type 2 (DD2).

Spezifität

Cross-reacts with OCRL/INPP5b (Prof. J. Trimmer, Univerity of California, Davis).

Immunogen

Recombinant protein corresponding to human OCRL/INPP5b.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Entwicklungsabhängige Signalübertragung

Detect Inositol polyphosphate 5-phosphatase OCRL-1 using this mouse monoclonal antibody, Anti-OCRL/INPP5b Antibody, clone N166A/26 validated for use in western blotting & IHC.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected OCRL/INPP5b in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected OCRL/INPP5b in COS cell lysates transiently transfected with GFP-tagged OCRL or RFP-tagged INPP5b, and demonstrated a loss of signal in COS cell lysates transiently transfected with untagged Kv2.1 plasmids (Prof. J. Trimmer, University of California, Davis.).
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OCRL/INPP5b in human cerebellum and human cardiac myocytes.

Qualität

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected OCRL/INPP5b in 10 µg of human brain tissue lysate.

Zielbeschreibung

~104 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Rat brain tissue lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable